The Blog

The Best Kit For Solubility Screening

Posted on 16. Feb, 2015 by Beryl Dalton in Marketing

Protein aggregation affects their availability for certain functions. This calls for solubility screening to establish the formulations or conditions that will protect your proteins. The choice of kit will determine the comprehensiveness or intensity of the results you obtain. The protein samples are solubilized during testing depending on expectations.

The kit allows you to use salt, additives and PH to detect quick aggregation capabilities or properties. This kit allows testing based on several conditions in parallel and label free forms. You are not required to use centrifuge during the testing. Simple mixing and spinning produces the desired results. This means that you can obtain desired results in different circumstances.

The testing kit enables you to rescue protein samples that are in reversible form. This form of testing prevents errors that occur when the samples aggregate. These proteins are therefore available for multiple tests. The kit comes with a detailed dashboard that make interpretation of results easier because it gives a detailed trend that is easy to relate with what is in the market.

Protein aggregation hinders availability of proteins for certain functions and is therefore undesirable. The main causes of aggregation include prolonged storage, vigorous stirring, elevated temperatures and the presence of ligands or protein binding partners. The best way to avoid aggregation is to make the right decision on PH, stabilization additives and salt.

With a PH range between 3 and 10, the screening kit offer sufficient buffer. It also comes with solubility enhancers such as sugars, salts, amino acids, reducing agents and polyols, among others. The results are clear and precise which allows you to identify aggregating and non-aggregating proteins.

A single label free experiment can be used to explain over ninety formulations. The control tests are six with yes and no possibilities. A basic test is where soluble proteins go through the filters while insoluble proteins do not. This is a clear indication of the properties of these proteins that can be used to draw inferences and make conclusions.

Protein solubilization tests give the conditions under which your proteins will not aggregate. From such tests, it is observed that filtration processes yield proteins when they are in soluble form. They are available in aggregated form if the test does not show any traces of proteins.

Screening results will point at the conditions in which the proteins exist. The results allow you to identify well behaved proteins that will serve your intended purpose. This confirmation is necessary if expected results are to be obtained when such proteins are used in large scale.

Testing kits are beneficial compared to the other methods available in the industry. They give clear results that can be interpreted based on the data available in the protein field. It is fast and convenient taking an hour to get desired results. Those results are reliable compared to the trial and error approach by other methods.

The experiment does not consume large amounts of proteins and is therefore ideal for large scale testing. Reduction in protein use allows it to be matched with assay agents. A detailed report allows more conclusions and inferences to be drawn. There is no restriction on the assay that can be used.

This online page has up-to-date information on solubility screening that people need to know about. To learn more, visit the relevant website on http://www.freeslate.com right now.

Beryl Dalton

More Posts

Tags: business, leadership, Marketing, news, sales

About Beryl Dalton

View all posts by Beryl Dalton →